We conclude that the gene we studied is a new loci required for efficient nitrogen fixation and for competitive nodulation of soybeans by bradyrhizobium japonicum strain gx201 我們認(rèn)為在突變體菌株gx217中tn5gusa5所插入的開放閱讀框架是一個新的與競爭結(jié)瘤有關(guān)的基因。
Plant assay was performed with the wild - type strain gx201 , the mutant strain gx217 and the function recovered strain rgx217 . the results indicate that the mutant strain gx217 showed a day delay in nodulation time and its abilities of nodule formation efficiency and competitive nodulation were apparently decreased compared with the parental strain gx201 respectively 通過對野生型菌株gx201 ,突變體菌株gx217及其功能互補菌株rgx217進行植株試驗,發(fā)現(xiàn)突變體菌株gx217在結(jié)瘤時間方面較野生型菌株gx201慢一天,其結(jié)瘤效率及競爭結(jié)瘤能力均比gx201明顯降低。
Genetic diversity and phylogeny of 55 slow - growing rhizobia isolated from peanut ( arachis hypogaea ) in china were determined by analysis of host - plant range , phynotype , 16s rrna rflp , 16s rrna sequence , 16s - 23s igs rflp , rapd , rep - pcr , dna - dna hybridization homology . at the same time , the competitive nodulation capacity of rhizobia , effect of host plants and soil ph on the rhizobia were determined for screening and improvement of high effective rhizobium inoculant 本研究采用宿主范圍試驗、表型性狀測定、 16srrna - rflp 、 16srrna序列分析、 16s - 23srdnaigsrflp分析、 rapd分析、 rep - pcr分析和dna - dna同源性分析等技術(shù)系統(tǒng)研究了從我國不同地域分離的55株花生根瘤菌的遺傳多樣性及其在根瘤菌系統(tǒng)發(fā)育中的地位和相互關(guān)系。